| Component | Amount per reaction (µL) |
Final amount/concentration |
|---|---|---|
| Water | 9.5 | - |
| 5× Phusion HF Buffer | 10 | 1× |
| 10 mM dNTPs | 2.5 | 500 µM |
| Primer A (25 µM) | 1 | 0.5 µM |
| Primer B (25 µM) | 1 | 0.5 µM |
| Phusion DNA Polymerase (2000 U/mL) | 1 | 2 U |
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