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. 2017 Aug 29;17:206. doi: 10.1186/s12862-017-1051-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — In vitro assay for protein-protein interaction between ASPM IQ motif and calmodulin (CaM). a GST pull-down assay indicating a possible interaction of the ASPM IQ motif and CaM. Latin name of species shorthand initial capital letter for the genus and the first three letters of species listed upper gel-image. b Quantitative analysis of interaction of ASPM IQ and CaM using BLI analysis. The association and dissociation of increasing concentrations of ASPM IQ motif to CaM were shown. c Comparison of dissociation constants (Kd) values derived from BLI analysis. P values were calculated by Student’s t-test