. 2017 Aug 29;6:e27441. doi: 10.7554/eLife.27441

Table 1. Fractions and molecular properties of diffusional particles of Syt1^C2AB generated various conditions as measured by fluorescence correlation spectroscopy.

Averages and standard errors from three independent trials are shown.

Proteins	Total protein conc. (nM)	Mg²⁺ + polyphosphate	Readout channel	Component 1			Component 2
Proteins	Total protein conc. (nM)	Mg²⁺ + polyphosphate	Readout channel	Fraction (%)	Diffusion time (µs)	Diffusion coefficient (μm²/s)	Fraction (%)	Diffusion time (µs)	Diffusion coefficient (μm²/s)
Syt1-Alexa488	~30	No	Alexa488	100	620 ± 35	70 ± 4	n/a	n/a	n/a
Syt1-Alexa647	~30	No	Alexa647	100	616 ± 22	70 ± 3	n/a	n/a	n/a
Syt1-Alexa488 + Syt1-Alexa647 + unlabeled Syt1	~5000	No	Alexa488	100	583 ± 20	74 ± 8	n/a	n/a	n/a
Syt1-Alexa488 + Syt1-Alexa647 + unlabeled Syt1	~5000	No	Alexa647	100	577 ± 20	75 ± 8	n/a	n/a	n/a
Syt1-Alexa488 + Syt1-Alexa647 + unlabeled Syt1	~5000	Yes	Alexa488	77 ± 2	527 ± 20	82 ± 4	23 ± 2	4168 ± 194	10 ± 1
Syt1-Alexa488 + Syt1-Alexa647 + unlabeled Syt1	~5000	Yes	Alexa647	76 ± 2	567 ± 26	77 ± 4	24 ± 2	3862 ± 196	11 ± 1

Table 1. Fractions and molecular properties of diffusional particles of Syt1C2AB generated various conditions as measured by fluorescence correlation spectroscopy.