Figure 4.

Strategy for quantitation of mtDNA by Southern blot analysis. (A) The ScaI and BglII maps of mtDNA and pXLM32 are shown to indicate how these two DNAs yield fragments of different sizes that contain the entire extent of the 1.8 kb HindIII fragment used as a hybridization probe. The pBR322 vector in pXLM32 is shown as a thick line. The HindIII sites are shown only in the pXLM32 diagram. (B) A representative standard curve showing the relationship between phosphorimager intensity and quantity of pXLM32 DNA. The line drawn through the data points is a linear regression.