Figure 3. Runx3 independent impact of Thpok and LRF on Treg function.

(A) Immunoblot analysis of Runx protein expression in sorted CD44^lo CD25⁻ naive CD4⁺ and CD8⁺ cells from WT mice and in YFP⁺ Tregs from control, Thpok KO or dKO male mice. Each lane is from 10⁵ cells, and expression of β-actin is shown as a loading control. Data is representative of two biological replicates for each sample.

(B) Survival curve of control (grey line) or Thpok^fl/flLrf^fl/fl Runx3^fl/fl (black line) Foxp3^YFP-Cre male mice. Number of mice is indicated in plot. Median survival for Foxp3^YFP-Cre Thpok^fl/flLrf^fl/fl Runx3^fl/fl is 50 days. Data from control mice is the same as in Fig. 2B and shown for comparison purposes.

(C) CD8 vs. CD4 expression is shown on Foxp3⁺ T cells from control or Thpok^fl/fl Lrf^fl/fl Runx3^fl/flFoxp3^YFP-Cre male mice. Data is representative of three mice of each genotype analyzed in three independent experiments.