Figure 2.

RV16 replication was significantly enhanced in monocytes in the presence of CM from epithelial cells. In the presence and absence of CM, monocytes were infected with RV16 as indicated and were then harvested for analysis after thorough washing with PBS. (A) RV16 mRNA was measured by qPCR analysis in THP-1 cells after RV infection for 24 h. (B) RV16 coat proteins were analyzed by Western blot analysis. Actin was used as a loading control. Biological duplicates are shown. Arrows represent viral coat proteins: VP0 (upper band) and VP4 (lower band). (C) Cells were infected for 24 h and thoroughly washed with PBS. Then, media were collected at 6 h, 24 h, and 48 h. Infectious viral particles were measured using a modified 50% tissue culture infective dose (TCID₅₀) assay. (D) RV16 mRNA was measured by qPCR analysis in THP-1 cells in the presence of CM from primary HBE cells for 24 h. *P < 0.05 RV versus control; ^$P < 0.05 CM (or CMp) versus BM. BM, base media; CM, conditioned media from A549 cells; CMp, conditioned media from primary HBE cells; HBE, human bronchial epithelial.