Figure 4.

ICAM1 was highly elevated in both cocultivated and CM-treated monocytes. (A) THP-1 cells were cocultivated with A549 cells, and samples were harvested as described in Figure 1. ICAM1 mRNA THP-1 cells were measured by qPCR analysis. (B) In the presence (CM) or absence (BM) of A549 CM, THP-1 cells were infected with RV16. ICAM1 mRNA was measured by qPCR analysis. (C) ICAM1 protein was measured by Western blot analysis. Actin was used as a loading control. Biological duplicates are shown. (D) Primary PBMC cells were treated with CM or BM, and ICAM1 mRNA expression was determined by qPCR. (E) ICAM1 protein in primary PBMC cells was measured by Western blot analysis. Actin was used as a loading control. Biological duplicates are shown. (F) CM from primary HBE cells (CMp) were used. ICAM1 mRNA in THP-1 cells was measured by qPCR analysis. (G) ICAM1 protein in THP-1 cells treated with CMp or BM were measured by Western blot analysis. ^$P < 0.05 CC versus IC, CM versus BM, or CMp versus BM. ICAM1, intercellular adhesion molecule 1.