Table 1.

Conditions and Set Points for the Ultra-Performance Liquid Chromatography

Condition	Set point
Mobile phase A	2 mM ammonium formate, pH 4.8
Mobile phase B	Methanol
Mobile phase ratio	30:70 A:B
Column	Waters HSS C18 50×2.1 mm, 1.8 μm
Flow rate	0.5 mL/min
Wavelength	222 nm
Column temperature	50°C
Injection volume	10 μL

HSS, high-strength silica.