Table 2.
Settings for the Tandem Quadrupole Mass Spectrometry and Multiple Reaction Monitoring
| TQD MS/MS | |
|---|---|
| Ionization mode | ESI positive |
| Capillary voltage | 3.5 kV |
| Cone voltage | 30–35 V |
| Desolvation gas temperature | 250°C |
| Desolvation gas flow | 500 L/h |
| Source temperature | 150°C |
| Cone gas flow | 50 L/h |
| Mass scan range | 150–650 Da |
| MRM | |||||
|---|---|---|---|---|---|
| Precursor ion (m/z) | Product ion (m/z) | Dwell time (sec) | Cone voltage (V) | Collision energy (V) | Use |
| 315.2 | 193.2 | 0.200 | 35 | 20 | Quantifier |
| 315.2 | 259.3 | 0.200 | 35 | 20 | Qualifier |
ESI, electrospray ionization; MRM, multiple reaction monitoring; TQD MS/MS, tandem quadrupole mass spectrometry.