Fig 1. Parental allele-specific methylation of the MEG3 IG DMR and the MEST promoter.

Mean methylation levels and standard deviations of the paternal vs. maternal alleles were determined by DBS with the Roche GSJunior in FCB, AB, and VAT. For both genes, the non-imprinted allele, which is expected to be completely unmethylated, showed an aberrantly high methylation in all analyzed tissues, whereas the imprinted allele showed the expected (90–100%) methylation.