Fig 3. Epimutation rates of the MEG3 IG DMR and the MEST promoter in individual samples.
The percentage of paternal (black dots) versus maternal alleles (gray dots) with >50% aberrantly (de)methylated CpGs was determined by DBS with the Roche GSJunior in individual FCB, AB, and VAT samples. With very few exceptions, the unmethylated alleles of the paternally imprinted MEG3 and the maternally imprinted MEST genes displayed much higher epimutation rates than the methylated alleles.