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. 2017 Aug 30;12(8):e0184110. doi: 10.1371/journal.pone.0184110

Fig 3. uPA expression was upregulated by Gemcitabine treatment and resulted in promotion on sphere formation, invasion, and colony formation.

Fig 3

(A) GRP78 protein level was measured for determine whether it is modulated by gemcitabine treatment. (B) Upregulation of uPA by gemcitabine-induced GRP78. (C) and (D)Transwell assay for analyzing the effects of uPA or GRP78 on invasive capacity of Panc-1 CSCs. (E) Sphere formation assay for determining the effect of uPA. (F) Soft agar assay was performed for determining the effect of uPA on colony formation ability in Panc-1 CSCs. (G) Percentage of cell phase was detected by PI staining. (H) The effects of uPA on Gemcitabine sensitivity in Panc-1 CSCs. *P<0.05.