Table 1. Primers sequences and efficiencies used for quantitative RT-PCR.
Gene | Forward primer | Reverse primer | E |
---|---|---|---|
BMP2 | 5’-CATCACGAAGAAGCCGTGGA-3’ | 3’-TGAGAAACTCGTCACTGGGG-5’ | 1,96 |
ID1 | 5’-GGTGGTACTTGGTCTGTCGG-3’ | 3’-CCTTGCTCACTTTGCGGTTC-5’ | 1,88 |
AlK3 | 5’-TGAGACAGCAGGACCAGTCA-3’ | 3’-GATTCTGCCCTTGAACATGAGA-5’ | 1,92 |
18S | 5’-CATTCGAACGTCTGCCCTATC-3’ | 3’-CTCCCTCTCCGGAATCGAAC-5’ | 1,99 |
GAPDH | 5’-TGACGTGCCGCCTGGAGAAA-3’ | 3’-AGTGTAGCCCAAGATGCCCTTCAG-5’ | 1,99 |