Figure 2.

Molecular mass estimation by SEC–MALS under native conditions of 6-helix and 5-helix constructs in the presence or absence of excess C34 peptide. (A) Injection of 200 μg of 6-helix alone (red) and with a 5-fold molar excess of C34 (blue). (B) Injection of 200 μg of 5-helix alone (green) and with a 5-molar excess of C34 (black). Observed masses and compositions are indicated beside the peaks. Experiments were conducted in 10 mM Tris-HCl (pH 7.6) and 150 mM NaCl.