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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Stem Cells. 2014 Jan;32(1):105–115. doi: 10.1002/stem.1513

Figure 5.

Figure 5

Effect of inhibiting Ryk on Wnt5a-mediated hematopoietic repopulation by ex vivo expanded lin−, Sca-1+, c-kit+ (LSK), CD150+, CD48− cells. (A, Left): Raw data of hematopoietic chimerism of peripheral blood cells 4 weeks after transplant of cultured LSK, CD150+, CD48− cells. Each point represents a single recipient. Average percent contribution by cultured cells (% CD45.2+, GFP−) for each condition is marked with a horizontal bar. The number of engrafted recipients (defined as >1% chimerism) and the total number of transplanted recipients are shown for each condition. (A, Right): Bar graph showing average percentage of hematopoietic chimerism of peripheral blood cells. Only recipients with >1% chimerism are included. Group sizes are control, n=9; rWnt5a, n=8; rWnt5a+α-Ryk, n=4; α-Ryk, n=3. Data are pooled from two independent experiments. (B, Left): Raw data of hematopoietic chimerism of peripheral blood cells 16 weeks after transplant of cultured LSK, CD150+, CD48− cells. Average percent contribution by cultured cells (% CD45.2+, GFP−) for each condition is marked with a horizontal bar. The number of engrafted recipients (defined as >1% chimerism) and the total number of transplanted recipients are shown for each condition. (B, Right): Bar graph showing average percentage of hematopoietic chimerism of peripheral blood cells. Group sizes are control, n=7; rWnt5a, n=6; rWnt5a+α-Ryk, n=3; α-Ryk, n=4. Only recipients with >1% chimerism are included. Data are pooled from two independent experiments. (C): Average percentage of CD45.2+, GFP− peripheral blood cells of myeloid (left) or lymphoid lineage (right) in recipients 16 weeks after transplantation of cells cultured under control conditions or with rWnt5a. Myeloid engraftment is the percentage of CD45.2+, GFP− cells that are positive for the cell surface markers Gr-1, CD11b, and Ter-119. Lymphoid engraftment is the percentage of CD45.2+, GFP− cells that are positive for the cell surface markers CD3 and B220. For all panels, error bars represent SD and p values between control versus rWnt5a groups were determined using paired t test. Statistical analysis of engraftment frequencies between control groups and other experimental groups were determined using Fisher’s exact probability test (*, p<.05; **, p<.01). Abbreviations: GFP, green fluorescent protein; Ryk, related to receptor tyrosine kinase; rWnt5a, recombinant Wnt5a.