Table S1.

Aptamer sequences used in the assays

Aptamer	Conjugation substrate	Sequence
MB-TBA-15	Gold film	5′ S-(CH2)6-/MB/iSp18/TTTTTG GTT GGT GTG GTT GG -3′
		iSp18 is a hexa-ethyleneglycol spacer for increasing flexibility and density of aptamer on the gold film (24)
TBA-29	60 nm AuNP	5′ S-(CH2)6-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′
TNF-α	60 nm AuNP	5′ S-(CH2)6-ATC CAG AGT GAC GCA GCATGC TTA AGG GGG GGG CGG GTT AAG GGA GTG GGG AGG GAG CTG GTG TGG ACA CGGTGG CTT AGT -3′

Oligonucleotide sequences are shown 5′–3′. MB-TBA-15 and TBA-29 were used in the assays for the detection of thrombin. MB-TBA-15 aptamers were immobilized onto gold film, and TBA-29 aptamers were immobilized on AuNP. TNF-α aptamers were immobilized onto AuNP for the TNF-α assays.