Fig. 2.
Stomatal movement and water transport responses of Col-0, pip2;1-1, pip2;1-2, pip2;1-PIP2;1 to flg22. (A) Epidermal peels from the indicated genotypes were placed in a bathing solution for 3 h under light and further incubated in the absence (white bars) or in the presence of 1 µM flg22 (green bars). Stomatal aperture was measured after 2 h. Data from three independent plant cultures, each with 60 stomata per genotype. Error bars represent SEs. The letters indicate statistically different values (ANOVA, Newman–Keuls: P < 0.05). (B) Guard cell protoplasts were isolated from the indicated genotypes and incubated under light in the absence (white bars) or presence (green bars) of 1 μM flg22. Their Pf was measured as described in Materials and Methods. Data from three independent experiments, with a total of n = 12–17 protoplasts per condition. Same conventions as in A.