Fig. 3.

Influx of exogenously supplied H₂O₂in guard cells of Col-0, pip2;1-1, and pip2;1-2 plants. Epidermal peels from Col-0 (A and D), pip2;1-1 (B and E) or pip2;1-2 (C and F) plants were placed under light for 3 h and subsequently treated for 6 min by flg22 (1 µM) (green) or water (yellow) (A–C) or by ABA (10 µM) (black) or ethanol (0.02%) (yellow) (D–F) before application of exogenous H₂O₂. Kinetic changes in HyPer fluorescence (R/R₀) were recorded before and after the application of 100 µM H₂O₂ (red arrow at t = 5 s). Error bars represent the SEs from measurements cumulating three independent plant cultures, with a total between 30 and 40 guard cells per genotype.