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Kinetic variations of HyPer signal induced by exogenous flg22 in mesophyll cell protoplasts of Col-0 and pip2;1-2. Protoplasts from Col-0 (blue diamonds) and pip2;1-2 (tan triangles) plants were exposed to light during 2 h before exposure at t = 0 to flg22 (1 µM). Ratiometric fluorescence (R) of HyPer with excitations at 475 and 438 nm was measured at the indicated time. Error bars represent SEs. Data are from at least 50 mesophyll protoplasts per genotype and experiment.
