Skip to main content
. 2017 Aug 8;114(34):E7121–E7130. doi: 10.1073/pnas.1704999114

Fig. 6.

Fig. 6.

A schematic representation of the mechanism by which 20E regulates PKCδ expression and EcRB1 phosphorylation. (1) 20E via ErGPCR1 and ErGPCR2, Gαq, PLC, calcium ion, and CaMKII signaling triggers USP1 phosphorylation and acetylation to modulate formation of EcRB1/USP1 transcription complex to bind on EcRE for 20E pathway gene transcription based on our previous studies (16, 36). (2) 20E rapidly up-regulates PKCδ expression via EcRB1 and USP1. The overexpressed PKCδ catalytic domain was autophosphorylated and localized to the nucleus to phosphorylate EcRB1 at T468. (3) The phosphorylated EcRB1 interacts with P-USP1-Ac to form the EcRB1/USP1 transcriptional complex to promote apoptotic gene expression and apoptosis. Through this positive feedback mechanism, 20E promotes apoptosis during insect metamorphosis.