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. 2017 Aug 8;114(34):E7121–E7130. doi: 10.1073/pnas.1704999114

Fig. S4.

Fig. S4.

PKCδ knockdown via RNAi in larvae restrains wing imaginal disk growth and promotes apoptotic gene expression in wing disk. (A) 20E promotes fore wing disk growth. Fore wing imaginal disks were separated and cultured in Grace’s medium with 20E for 48 h. The wing disk length was measured using ocular micrometer. 20E used a lower concentration (1 μM) and a higher concentration (5 μM). DMSO was used as a solvent control. Each bar represents the mean of 10 disks. Bars are mean ± SD. (B) Morphology of fore wing imaginal disk after injection of dsRNA. The fresh fore wing disks were separated under microscope for observation after two injections of dsRNA for 80 h. dsGFP served as a control experiment. (C) The length of wing imaginal disk after PKCδ knockdown. The length of fore wing disks was measured using ocular micrometer after the disks were separated. Each bar represents the mean of six disks. Significant differences were calculated using Student’s t test (*P < 0.05). (D and E) Detection of apoptosis-related genes using qRT-PCR in the wing disk and midgut after the second dsPKCδ or dsGFP injection for 80 h. The wing disk and midgut were separated from the same individual. The values are the mean ± SD. Significant differences were calculated using Student’s t test (*P < 0.05). Three biological replicates and three technical replicates were performed for the qRT-PCR.