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Identification of putative SfU6 promoters and successful CRISPR-Cas9 editing of Sf-fdl. (A) Multiple sequence alignment of BmU6-2 promoter and SfU6 promoter candidates. (B) CEL-I nuclease assay results obtained using genomic DNA from Sf9 cells edited with CRISPR-Cas9 vectors encoding Sf-fdl targeting sequences (Table S1) under the control of the BmU6-2 or SfU6-3 promoters.
