Fig. 3.

Identification of putative TnU6 promoters and successful CRISPR-Cas9 editing of Tn-fdl. (A) Multiple sequence alignment of SfU6 promoter and TnU6 promoter candidates. (B) Diagram showing Tn-fdl gene structure and highlighting specific Cas9 targeting sequences and PCR primer sites. (C) CEL-I nuclease assay results obtained using genomic DNA from High Five cells edited with CRISPR-Cas9 vectors encoding a Tn-fdl targeting sequence (Table S1) under the control of the DmU6:96Ab, BmU6-2, SfU6, or TnU6 promoters.