Fig. 3.

Transient inhibition of reverse transcription RNAseH activity results in a delay of capsid integrity loss. (A) CsA washout of CHO-TRIMCypA cells in the absence of 7390 (black) and with 7390 washed out for all wells at 105 min (gray). (B) ∆t for infection of CHO cells incubated and washed with DMSO mock (Left) and 7390 (Right); the capsid integrity loss was delayed in accordance with 7390 removal (50 min postaverage fusion of all particles). Medians are shown as gray horizontal bars. (C) Mean particle intensity of GFP signal (green) and mCherryVpr (red) in the presence of 7390, followed by its washout (arrow) 50 min postfusion. STDEV, standard deviation. *P < 0.5.