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(A) Immunoblot of whole-cell extracts of CHO pgsa745 cells expressing HA-TRIMCypA or transduced with a control (Ctrl) vector. CHO TCypA, CHO-TRIMCypA. (B) TRIMCypA effectively destroys incoming HIV-iGFP-mCherryVpr particles when comparing CHO-TRIMCypA cells to CHO-TRIMCypA cells where the proteasome was inhibited by MG132 as measured by time-lapse imaging. Error bars represent SEM.
