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. 2017 Aug 7;114(34):E7140–E7149. doi: 10.1073/pnas.1706833114

Fig. S2.

Fig. S2.

IFN-γ expression in BM-resident lymphocytes. FACS analysis of IFN-γ production by CD8+ T (A and B), CD4+ T (C and D), and NK (E) cells isolated from miR-146+/+, miR-146a−/−, miR-146a−/−Traf6+/− BM. BM cells harvested from 8-wk-old female mice were analyzed by flow cytometry for intracellular IFN-γ expression. Representative FACS plots showing IFN-γ expression in CD8+ T (A), CD4+ T (C), and NK (E) cells from miR-146+/+, miR-146a−/−, and miR-146a−/−Traf6−/+ BM. Quantification of intracellular IFN-γ expression in stimulated CD8+ (B) and CD4+ (D) T cells from miR-146+/+, miR-146a−/−, and miR-146a−/−Traf6+/− BM. IFN-γ MFI values were calculated using the FlowJo X software and then were normalized to miR-146+/+ samples. Data are representative of at least three different experiments with n ≥ 3 for each genotype. Data are shown as mean ± SD. P values were calculated using standard one-way ANOVA tests. *P ≤ 0.05; ns, not significant.