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Synthesis of blue fluorescent probe (7HC) labeled JD with hybrid RHF chains. Reagents and conditions: (i) HOSu, EDC, DMF, 0 °C for 1 h, then 23 °C for 12 h; (ii) N,N-diisopropylethylamine (DIPEA), DMF, 23 °C, 12 h; (iii) 4-(dimethylamino)pyridinium 4-toluenesulfonate, DCC, DCM, 23 °C for 12 h; and (iv) CuSO4·5H2O, sodium ascorbate, THF, water, 23 °C for 12 h.
