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. 2017 Jul 24;114(34):9110–9115. doi: 10.1073/pnas.1708492114

Fig. 4.

Fig. 4.

Contact site morphologies after Ca2+-triggered fusion. (A) Gray scale tomographic 2D-slice view of SVCpx + PM vesicles after incubation with 500 μM Ca2+ for 1 min. (B–F) Corresponding 2D-slice views (gray, Left, tilt axes along the solid white lines) and isosurface representations (dark pink, Right) of subtomograms of the vesicles. (G) Gray scale tomographic 2D-slice view of SVCpx + PMC1C2BMUN vesicles after incubation with 500 μM Ca2+ for 1 min. We also observed a substantial increase in the number of C1C2BMUN fragments that were bound to PM vesicles relative to Fig. 3C, consistent with an increase of membrane binding of the C2B domain in the presence of Ca2+ (26). (H–J) Corresponding 2D-slice views (gray, Left, tilt axes along the solid white lines) and isosurface representations (dark blue, Right) of subtomograms of the vesicles. Videos of representative tomograms are available in Movies S5 and S6. Percentages were calculated with respect to the sum of the total number of observed interfaces plus the number of vesicles without interfaces (Table S1). Tomogram 2D slice thickness: 1 pixel = 3.42 Å. (Scale bars: black, 100 nm; gray, 25 nm.) Thin white lines, tilt axes.