Fig. S7.

Image processing steps: (A) region of interest (ROI) detection, (B) cell trap detection, (C) precise ROI detection using the dot barcode, (D) empty trap detection [empty cell trap (I), occupied cell trap (II), template for empty trap averages (III), average empty trap (IV)], and (E) background removal and cell pole detection [occupied cell trap (I), average empty trap (II), enhanced contrast of the occupied trap (III), profile of intensity changes through the contrast enhanced occupied cell trap image (IV)].