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SH3P2 does not affect AMSH3 DUB activity. DUB assay using K63-linked diubiquitin-TAMRA as a substrate. Recombinant AMSH3 was incubated with diubiquitin-TAMRA with or without preincubation with an equimolar amount of SH3P2. Fluorescence was measured every minute (black, diubiquitin-TAMRA with AMSH3; red, diubiquitin-TAMRA with AMSH3 and SH3P2; gray, K63-linked diubiquitin-TAMRA only).
