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. 2017 Aug 7;114(34):9104–9109. doi: 10.1073/pnas.1711645114

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Fig. S3. — Amplitude AFM images of Het-s protofibril/fibril formation over time in the absence and presence of GroEL. (A) Het-s(218-29) fibrils (100 μM in monomer) formed after 2 wk at room temperature. Het-s(218–289) protofibrils and fibrils (100 μM in monomer) obtained immediately (B), after 18 h (C), and after 2 wk (D) following the addition of 370 μM (in subunits) GroEL. In B and C, only protofibils are present; but after 2 wk (C), both fibrils and profibrils are clearly observed. Fibrilization was carried out at room temperature under quiescent conditions.