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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Nature. 2017 Jun 22;547(7664):463–467. doi: 10.1038/nature23267

Figure 3. H3K9me states regulate the recruitment of HP1 proteins and Clr4.

Figure 3

a–c, Swi6 (a), Flag-Chp2 (b), and Flag-Clr3 (c) ChIP-seq reads mapped to pericentromeric repeats on the right arm of chromosome 1. Arrowheads indicate the location of primers for ChIP-qPCR analysis presented in Extended Data Fig. 9. d, Quantitative MS analysis of the association of chromo domain proteins with differentially methylated H3K9 peptides. e, Silver stain (top) and western blot (bottom) of proteins isolated by H3 tail peptides. For gel source data, see Supplementary Figure 1. f, Quantification of TMT-labeled peptides for the indicated protein. Error bars, s.d.; n = 3 biological replicates for me2 and me3 peptides. H3K9me3 (g), H3K9me2 (h), and Flag-Clr4 (i) ChIP-seq reads mapped as described for panels a–c.