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. Author manuscript; available in PMC: 2018 Aug 2.
Published in final edited form as: J Am Chem Soc. 2017 Jul 24;139(30):10403–10409. doi: 10.1021/jacs.7b04830

Figure 1.

Figure 1

Benenodin-1 gene cluster and sequence. A: The gene cluster for lasso peptide benenodin-1 showing the highly conserved architecture typical of lasso peptides produced in alpha-proteobacteria. The A, B, and C genes are required for lasso peptide biosynthesis while the E gene encodes lasso peptide isopeptidase. B: Sequence of benenodin-1 aligned with astexin-3. The conserved threonine in the leader peptide is colored orange, the isopeptide-bonded glycine is colored blue, and the isopeptide-bonded aspartic acid is colored red. C: Engineered gene cluster for the heterologous expression of benenodin-1 in E. coli.