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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Nat Protoc. 2016 Dec 1;12(1):15–31. doi: 10.1038/nprot.2016.153

Figure 3.

Figure 3

Cell culture density and phenotype from pluripotency to mesoderm. (a) Different cell densities tested throughout this protocol to assess density dependency of differentiation into cardiac and endothelial lineages. (b,c) Phenotypic changes that are observed as cells transition from pluripotency to day 1, remaining in close contact during exposure to activin A, (b) and then undergo near single-cell dispersion across the plate during exposure to BMP4 and progression to mesoderm on day 2 (c). Scale bars, 500 μm.