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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Nat Protoc. 2016 Dec 1;12(1):15–31. doi: 10.1038/nprot.2016.153

Figure 4.

Figure 4

Cardiac directed differentiation. (a) Effect of cell density on cardiomyocyte differentiation efficiency based on FACS analyses of cTnT+ cells on day 14. (b) Immunohistochemistry of cardiomyocytes generated from high-density differentiation (159 cells cm−2) at day 14 based on staining for the transcription factor Nkx2-5, the myofilament protein α-actinin, and the intercalated disk protein connexin 43. n = 6 biological replicates per sample. The antobodies used are listed in the Materials section. Data are represented as mean ± sem. Scale bars, 100 μm.