Table 1.
Quantitative RT–PCR primers for human genes.
| Gene name | Forward primer | Reverse primer |
|---|---|---|
| Housekeeping | ||
| HPRT | TGACACTGGCAAAACAATGCA | GGTCCTTTTCACCAGCAAGCT |
| Pan mesoderm | ||
| T | CAAATCCTCATCCTCAGTTTG | GTCAGAATAGGTTGGAGAATTG |
| KDR | ATGCACGGCATCTGGGAATC | GTCACTGTCCTGCAAGTTGCTGTC |
| Cardiogenic mesoderm | ||
| NODAL | TGGAGGTGGGATGAAGTCACCTAT | AACCCAGCCTGAGGCAATGAGATT |
| DKK1 | AACAGCTATCCAAATGCAG | TCACAGGGGAGTTCCATAAA |
| MESP1 | TCGAAGTGGTTCCTTGGCAGAC | CCTCCTGCTTGCCTACAAAGTGTC |
| GSC | GAGGAGAAAGTGGAGGTCTGGTT | CTCTGATGAGGACCGCTTCTG |
| Hemogenic mesoderm | ||
| CDX1 | GGTGGCAGCGGTAAGACTC | TGTAACGGCTGTAATGAAACTCC |
| WNT8a | GCAGAGGCGGAACTGATCTT | CGACCCTCTGTGCCATAGATG |
| WNT3a | AACTACGTGGAGATCATGCCC | GACTCCCTGGTAGCTTTGTC |
| Cardiac progenitor cells | ||
| TMEM88 | GCTGCCTTCAATCTTCTCCTG | ATAAAGGGCTCGGCTGTAGG |
| GATA4 | ACACCCCAATCTCGATATGTTTG | GTTGCACAGATAGTGACCCGT |
| ISL1 | ATTTCCCTATGTGTTGGTTGC | CGTTCTTGCTGAAGCCGATG |
| NKX2.5 | CCAAGGACCCTAGAGCCGAA | ATAGGCGGGGTAGGCGTTAT |
| MYL4 | TCAAAGAGGCCTTTTCATTG | CGTCTCAAAGTCCAGCATCT |
| Cardiomyocytes | ||
| TNNT2 | TTCACCAAAGATCTGCTCCTCGCT | TTATTACTGGTGTGGAGTGGGTGTGG |
| MYH6 | CAAGTTGGAAGACGAGTGCT | ATGGGCCTCTTGTAGAGCTT |
| MYL7 | TCCAACGTCTTTTCCATGTT | TCTGTCCCATTGAGCTTCTC |
| TBX5 | GAACCACAAGATCACGCAATTA | ACACCATTCTCACACTGGTAT |
| ATP2A2 | ATGACAACCCACTGAGAAGAGAA | CGAAGGTCAGATTGGTCTCATATTT |
| RYR2 | AGAACTTACACACACGCGACCTG | CATCTCTAACCGGACCATACTGC |
| Pan endothelium | ||
| SCL | AAGGGCACAGCATCTGTAGTCA | AAGTCTTCAGCAGAGGGTCACGTA |
| CD34 | AAATCCTCTTCCTCTGAGGCTGGA | AAGAGGCAGCTGGTGATAAGGGTT |
| CD31 | ATCATTTCTAGCGCATGGCCTGGT | ATTTGTGGAGGGCGAGGTCATAGA |
| Endocardial endothelium | ||
| NFATC1 | GCATTTTCCTTGATCCCTGT | AGCAGCTTTAGGGTGCAAAT |
| Hemogenic endothelium | ||
| HAND1 | TCAAAGACGCACTCTTCCAC | GTGCAGCGACAAAAAGAAAA |
| G ATA 1 | CTTTCAGGTGTACCCATTGC | AAAGTCTCCAGGAAGCTGGT |
| RUNX1 | ATGTGGTCCTATTTAAGCCAGCCC | TCATCTGGCTGAAGACACCAGCTT |