Table 3.
Antibody information.
| Antibody | Application | Stage | Vendor (cat. no.) | Dilution | Conjugate |
|---|---|---|---|---|---|
| cTnT | IHC/FACS | Primary | Thermo | 1:100 | NA |
| SMA | FACS | Primary | Abcam | 1:50 | NA |
| KDR | FACS | NA | R&D | 1:6 | PE |
| CD34 | FACS | NA | BD | 1:5 | PerCP |
| CD43 | FACS | NA | BD | 1:5 | APC |
| CD235a | FACS | NA | BD | 1:37 | PECy-7 |
| CD31 | IHC | Primary | Abcam (28364) | 1:30 | NA |
| CD31 | FACS | NA | BD | 1:5 | FITC |
| VE-cadherin | IHC/FACS | NA | Abcam (7047) | 1:5 | PE |
| Phalloidin | IHC | NA | Invitrogen (A12379) | 1:100 | Alexa Fluor 488 |
| Mouse anti-goat | IHC/FACS | Secondary | Jackson | 1:200 | PE |
| Rabbit anti-donkey | IHC/FACS | Secondary | Jackson | 1:500 | APC |
| Goat anti-rabbit | IHC | Secondary | Invitrogen (A11011) | 1:100 | Alexa Fluor 568 |
| Isotypes | |||||
| Rabbit IgG | IHC/FACS | NA | Abcam | 1:100 | NA |
| Mouse IgG1 | IHC/FACS | NA | eBioscience | 1:100 | NA |
| PerCP IgG1 | FACS | NA | BD | 1:50 | NA |
| PECy-7 IgG1 | FACS | NA | BioLegend | 1:50 | NA |
| FITC IgG | FACS | NA | Abcam | 1:50 | NA |
NA, not applicable.