Table 4.

Volume calculations for collagen gel.

Reagent	Stock concentration	Final concentration	Volume
Rat tail collagen, type I	3 mg ml−1	2 mg ml−1	$V_{\text{COLLAGEN}}=\frac{2\times V_{\text{FINAL}}}{3}$
10× M199	10×	1×	$V_{\mathrm{M}199}=\frac{V_{\text{FINAL}}}{10}$
1 N NaOH	1 N	—	VNaOH = 0.025 × VCOLLAGEN
EGM	—	—	VEGM = VFINAL − VM199 − VNaOH − VCOLLAGEN