Table 4.
Volume calculations for collagen gel.
Reagent | Stock concentration | Final concentration | Volume | |
---|---|---|---|---|
Rat tail collagen, type I | 3 mg ml−1 | 2 mg ml−1 |
|
|
10× M199 | 10× | 1× |
|
|
1 N NaOH | 1 N | — | VNaOH = 0.025 × VCOLLAGEN | |
EGM | — | — | VEGM = VFINAL − VM199 − VNaOH − VCOLLAGEN |