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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Nat Protoc. 2016 Dec 1;12(1):15–31. doi: 10.1038/nprot.2016.153

Table 4.

Volume calculations for collagen gel.

Reagent Stock concentration Final concentration Volume
Rat tail collagen, type I 3 mg ml−1 2 mg ml−1
VCOLLAGEN=2×VFINAL3
10× M199 10×
VM199=VFINAL10
1 N NaOH 1 N VNaOH = 0.025 × VCOLLAGEN
EGM VEGM = VFINALVM199VNaOHVCOLLAGEN
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