Table 3.
Sample | Repair step | Substrate | DNA repair activity§ |
---|---|---|---|
Brain nuclear lysates | DNA Glycosylase | 5-hydroxyuracil in a bubble DNA | Yes* |
Ligase | Nick-1 | No (P=0.06) | |
Linearized plasmid | Yes* | ||
Brain mitochondrial lysates | DNA Glycosylase | 5-hydroxyuracil | Yes* |
Uracil | No | ||
AP endonuclease | Abasic site | No | |
Polymerase | Gap | No (p=0.056) | |
Ligase | Nick-1 | Yes* | |
Nick-2 | Yes* |
In the column#4 titled DNA repair activity, the symbol § indicates whether there is a significant decrease in repair activity in the AD samples relative to control samples. In each row within this column, * symbol indicates that the p-value is <0.05. in cases of no difference but with a trend we indicated the p-values. Nick-1 is a heteroduplex with a 5′-P and 3′OH containing nick, where as Nick-2 is a heteroduplex with a nick containing 3′- and a 5′-OH groups. Nick to is generated by gap filling activity by a polymerase on a gap substrate.