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. 2001 Jul 1;29(13):2706–2714. doi: 10.1093/nar/29.13.2706

Figure 5.

Figure 5

Figure 5

Template opening in the presence and absence of δTFIIS. Transcription initiation complexes were assembled in the presence and absence of δTFIIS on radiolabeled mutant AdML templates as indicated at the top of the figure. (A) Initiation complexes were assembled with AdML+23G (lanes 1–3), AdML+25G (lanes 4–6), AdML+26G (lanes 7–9), AdML+27G (lanes 10–12) and AdML+28G (lanes 13–15). The G residue creating the stall site is indicated by the arrow. (B) Initiation complexes were assembled with AdML+29G (lanes 1–3), AdML+31G (lanes 4–6), AdML+33G (lanes 7–9), AdML+35G (lanes 10–12). The reactions included nucleotides (60 µM ATP, 10 µM UTP, 10 µM CTP and 120 µM 3′-OMeGTP) at 30°C for 10 min. After this, potassium permanganate was added for 20 s and the reactions were processed as described in Materials and Methods. The last T residue before the stall site is indicated by the arrow (see also Fig. 1).