TABLE 1:

Quantification of binding and motility of beads coated with CENP-F fragments.

Construct	2592C (laser trap assay)	2873C (laser trap assay)	sfGFP-2592C	sfGFP-2592C + 100 mM KCl	sfGFP-2892C	sfGFP-2927C	sfGFP-3003C	sfGFP-2892CΔ111
Moved with growth	8	6	5	0	12	14	7	0
Moved with shortening	18	12	21	1	34	14	9	2
Total bound beads	60	44	36	14	60	29	27	10
Total beads	209	164	97	233	358	300	567	424
Fraction of beads binding	0.29	0.27	0.37	0.06	0.17	0.1	0.05	0.02