TABLE 2:
Oligonucleotides used in this study.
| Number | Name | Sequence |
|---|---|---|
| 1 | fw_Cenpf1_BamHI | ccccctcgaggtcgacggtatcgataagcttgataGGATCCGCGATGAGCTGGGC |
| 2 | rev_Cenpf1 | GCTGCAAAATACTTCATCTACACTGCAC |
| 3 | fw_cenpf2 | AGTCTGCAGGAGGAGAATCTGACCAGGAAAGAAACC |
| 4 | re_cenpf2_NotI | cggccgctctagaactagtggatcccccgggctgcgcggccgcTCACTGGACCTTACAGTTCT |
| 5 | Fw_GFP | CAAACCAGTGCAGTGTAGATGAAGTATTTTGCAGCatggtgagcaagggcgagga |
| 6 | rev_GFP | GTTTCTTTCCTGGTCAGATTCTCCTCCTGCAGACTcttgtacagctcgtccatgc |
| 7 | sfGFP_fwd | GGACATATGcgtaaaggcgaagagctgt |
| 8 | sfGFP_rev | CAAGGATCCtttgtacagttcatccataccatgc |
| 9 | 2892C_fwd | GCTGGATCCcaatctaaacaagattcccgagg |
| 10 | 2892_2927_3003C_rev | TTACTCGAGTGCGtcactggaccttacagttct |
| 11 | 2927C_fwd | GCTGGATCCaataaagcttcaggcaagagg |
| 12 | 3003C_fwd | GCTGGATCcctgcgaagaacaaccatg |
| 13 | 2927CD111_fwd | GCTGGATCCcaatctaaacaagattcccgagg |
| 14 | 2927CD111_rev | TTACTCGAGTGCGtcacaggatatatgggctagtc |
| 15 | CENP-F_CRISPR1 | GAAATTAATACGACTCACTATAGGGGGATGTCAGCAAACCCTAAGTTTTAGAGCTAGAAATAGC |
| 16 | CENP-F_CRISPR1 | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |