TABLE 2:
Plasmids used in this study.
| Plasmid | Details | Source |
|---|---|---|
| pBW1546 | pGEX-KG-KAN | Reider et al. (2009) |
| pBW1503 | pGEX-KG-KAN::Syp1(aa602-870) | Reider et al. (2009) |
| pBW2250 | pET28aC/U::Mid2(aa273-376) | This study |
| pBW2505 | pET28aC/U::mid2(aa273-376; W277A, Y278A) | This study |
| pBW2515 | pET28aC/U::mid2(aa273-376; D14A, E15A, Y16A) | This study |
| pBW2019 | pRS316::GFP-Snc1 | Quenneville et al. (2006) |
| pRS425 | 2μ, LEU2 | Laboratory plasmid |
| pBW1034 | pRS425::SYP1 (2μ, LEU2) | This study |
| pBW1916 | pET28aC/U::Snc1(aa1-93) | This study |
| pBW2326 | pET28aC/U::Snc1(aa1-27) | This study |
| pBW2336 | pET28aC/U::snc1(aa1-27; D8A, P9A, Y10A) | This study |
| pBW1973 | pET28aC/U::snc1(aa1-93; D8K, Y10A) | This study |
| pBW2330 | pET28aC/U::Snc1(aa63-93) | This study |
| pBW2439 | pET28aC/U::snc1(aa63-93; W86A, Y87A) | This study |
| pRS426 | 2μ, URA3 | Laboratory plasmid |
| pBW1446 | pRS426::Syp1 (2μ, URA3) | Reider et al. (2009) |
| pBW1562 | pRS426::Syp1 (aa1-601) (2μ, URA3) | Reider et al. (2009) |
| pBW1622 | pYEP352::Rom1 (2μ, URA3) | Ozaki et al. (1996) |