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. 2017 Sep 1;28(18):2449–2459. doi: 10.1091/mbc.E17-01-0024

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© 2017 Panday et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Activity of the HMO1 promoter depends on Tor1p and the IFHL site. (A) Region upstream of HMO1 ORF (black). The IFHL site (red) is centered ∼605 base pairs upstream of the HMO1 start codon and followed by three predicted Reb1p binding sites. Positions of amplicons used in ChIP shown below. (B) Activity of HMO1 promoter using lacZ reporter constructs. (C) Activity of HMO1 promoter using lacZ reporter constructs in which IFHL site is mutated. Activity is reported in Miller units. (D) HMO1 expression in WT and tor1Δ cells. The mRNA levels are reported relative to IPP1 and calculated using the ΔC_t method. Data represent mean and SD from three independent experiments.