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. 2017 Sep 1;28(18):2449–2459. doi: 10.1091/mbc.E17-01-0024

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© 2017 Panday et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 11: — Dynamics of Ifh1p, Crf1p, and RNA Pol II binding to the HMO1 gene. (A) ChIP using antibody to FLAG-tagged Ifh1p or Crf1p before (0 min) and after addition of galactose to induce DSB. Primers amplifying fragment Up1 were used. IC, input control; No, no antibody; IP, immunoprecipitation with anti-FLAG antibody. (B) ChIP using antibody to RNA Pol II before (0 min) and after addition of galactose. (C) ChIP using antibody to RNA Pol II before (0 min) and after addition of rapamycin. Primer pairs amplifying fragments upstream of the HMO1 ORF (Up1–Up3) or the ORF are identified between B and C. IC, input control; No, no antibody; IP, immunoprecipitation with anti–Pol II antibody. Data are representative of duplicate experiments.