FIGURE 14:

Correlation between Reb1p and Hmo1p occupancy. (A) Quantification by qRT-PCR of ChIP using antibody to FLAG-tagged Reb1p before (Ctrl, dark blue bar) and after induction of DSB by addition of galactose (gray bars). DNA repair was induced by addition of glucose (blue hatched bars). Data were normalized to corresponding input control for each time point. Primer pair amplifying region containing Reb1p sites (Up1) was used. (B) Quantification by qRT-PCR of ChIP using antibody to FLAG-tagged Reb1p before (dark blue bar) and after induction of HMO1 expression (light blue bars); HMO1 was under control of the strong GAL1 promoter. Data were normalized to corresponding input control for each time point. Primer pair–amplifying region containing Reb1p sites (Up1) was used.