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Binding of Hmo1p and Tor1p to the HMO1 gene is reduced after inhibition of mTORC1 by addition of rapamycin. Quantification by qRT-PCR of ChIP using antibody to FLAG-tagged Hmo1p (A) or Tor1p (B) after addition of rapamycin. Data were normalized to corresponding input control for each time point. Sites upstream of the HMO1 ORF (Up3–Up1, blue bars) or within the ORF (green bars) were monitored. Three independent experiments were performed. Error bars represent SD.
