Fig. 3.

53BP1 focus formation. (A) Bars indicate the mean ± standard error of 53BP1 focus-forming cells in three independent control and three independent Asciz^−/− primary MEF cultures under basal conditions (open bars), in response to 24-h aphidicolin treatment (black bars) and following 30 min recovery after ionizing radiation (grey bars). More detailed quantifications of foci/cell are shown in Supp. Fig. S2. (B) Subcelllular distribution of 53BP1 in cell fractionation assays. M601 is used as a mitochondrial marker, and tubulin as a cytosolic marker.