Skip to main content
. 2017 Apr 20;28(9):2607–2617. doi: 10.1681/ASN.2016060626

Figure 7.

Figure 7.

A normal allele of the human COQ2 gene but not a mutant allele (COQ2-S146A) rescued nephrocyte function in flies expressing Drosophila Coq2-IR. (A) Fluorescence micrographs showing uptake of ANF-RFP nephrocytes of 1-day postemergence adult flies. Left panels (MHC-ANF-RFP) show intracellular ANF-RFP fluorescence (red). Right panels (MHC-ANF-RFP Hand-GFP) show RFP (red) merged with GFP (green, mostly nuclear). Hand-GFP expression confirms pericardial nephrocyte cell identity. All flies are transgenic for Hand-GFP. Control flies carry the Dot-Gal4 driver but no RNAi construct. Coq2-IR flies carry Dot-Gal4 driving RNAi transgene silencing the endogenous Drosophila Coq2 gene expression, and where indicated, also a UAS-COQ2 (wild-type human COQ2 allele) or a UAS-COQ2-S146N (mutant human COQ2 allele).6 (B) Quantitation of RFP levels (expressed relative to control) in control versus Coq2-IR nephrocytes expressing normal COQ2 or mutant COQ2-S146N. For each genotype, 30 nephrocytes (six nephrocytes from each of five flies) were examined (*P<0.05).