CD-specific ILK knockout in mice. At PN day 25, Ilk+/+;Pkhd1-Cre (Ilk+/+ CD) and Ilkfl/fl;Pkhd1-Cre (Ilk−/− CD) mice were euthanized, and kidney sections were stained for ILK (red), DBA (green), and DAPI nuclear stain (blue). Left panels (A and C) are images of kidney sections that were stained for ILK expression (red). Right panels (B and D) are merged images in which ILK, DBA, and DAPI staining was superimposed. Top panels (A and B) show an Ilk+/+ CD mouse kidney section with normal ILK expression in a CD (arrow). Bottom panels (C and D) show that Ilk−/− CD mouse kidney sections lack ILK expression in the CDs. Scale bar=5 µm.